Microscopy from Scientific Research

Single-molecule imaging in live cells and application of single-particle tracking analysis at found optimal labeling conditions (100 nM CoA-Atto 565, 10 uM Sfp, 10 mM MgCl 2 , 20 min: efficiency 82 +/- 3%). A representative cell is shown. Gray scale: TIRF images; left: first frame of the acquired 100-frame movie; right: in yellow, tracks obtained from a single-particle tracking analysis on frames 1-50, superimposed on the 50th frame (see also Additional file 2 : Video S1, Additional file 3 : Video S2). Scale bars: 5 um

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