Line Plot from Scientific Research

Left: representative TIRF images of cells incubated with a reaction mix including different CoA-Atto 565 concentrations (20-min reactions, 5 μM Sfp). Each field of view shows a single transfected labeled cell surrounded by dyes non-specifically adsorbed to glass or other non-transfected cells (see the Methods section). Scale bar: 5 μm. Right: quantification of spot density for non-specifically adsorbed dyes using different CoA-Atto 565 concentrations in the reaction, at 1 and 5 μM of Sfp (mean ± SEM from 12–38 fields of view analyzed in two independent replicates). Spot density is measured after 20 min of reaction and extensive sample washing (see the Methods section).

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