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Open access visualization of Microscopy, Experimental Images, DIC Image, TIRF channel, Atto 565
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Examples of experimental images corresponding to the workflow steps depicted in Fig. 1 B, for some of the analyzed conditions reported in D . Each image column shows a single cell: DIC image (top); TIRF channel of the tested probe Atto 565 (middle, orange), TIRF image of the control probe Abb.635 (bottom, red). Eff.: efficiency estimated (for Atto 565 in varying conditions) or previously measured (for Abb.635 in fixed conditions).

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