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Fluorescent labeling efficiency obtained for Atto 565 at various concentrations of CoA-dye ([Atto 565]) and Sfp ([Sfp]), after 20 min of reaction and in presence of 10 mM of MgCl 2 (dots: mean, error bars: SEM estimates; 15–40 cells from 2 independent replicates analyzed for each condition). Scale bar: 5 μm. See also Additional file 1 : Tables S1 and S2
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