Gel from Scientific Research

Cells that lacked PFD2 were transformed with a plasmid that expressed PFD2-HA or an empty vector. Cells were grown on selective minimal medium that contained glycerol at 25 C to the logarithmic growth phase and shifted to 37 C for 4 h. Equal volume of the input and elution fractions of the immunoprecipitation experiment was separated on SDS-PAGE and analyzed by Western blot against specific antibodies. The experiment was repeated three times. neg. control (negative control) in the elution fraction indicates that the lysate was incubated with beads not coupled with antibodies. Uncropped blots are presented as source data in the Additional file 13.

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