Workflow from Scientific Research

Serial ET volumes alignment diagram. Serial ET volume alignment is mainly divided into two steps. First coarse alignment. It is based on feature matching and affine transformation, and uses multi-resolution images to achieve rough alignment of target structures. Second fine alignment. After the coarse alignment of the volumes, based on the clear texture structure of the high-magnification image, use block matching, BP-Elastic, and thin plate spline to obtain the fine-tuning deformation fields. Then, apply them to serial volumes to correct the alignment of target structures between adjacent volumes

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