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Growth and l -histidine production of C. glutamicum CgHis2 variants with different genomic modifications. C. glutamicum CgHis2 fasB pyk served as starting variant for the construction of various strains combining three or four genomic modifications, which exhibited significant increase in l -histidine production (yellow). pyk always refers to the pyk1 gene. C. glutamicum CgHis2 fasB cps served as starting variant for the construction of various strains with no improvement of product formation (Additional file 1 : Fig. S9). Data represent average values and standard deviation of three independent technical replicates

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