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HEK293T cells were transfected with pcDNA3.1 or pcDNA3.1-pre-miR-103a-2 plasmids together with pG5-luciferase and pBind-RXR/LBD plasmids. Cells were then treated with or without 9-cis-RA (10 –7 M) for 12 h. The value of the firefly luciferase activity was normalized to the renilla luciferase activity to yield the relative luciferase (Luc) activity. Data are presented as mean SEM ( n = 3), **** P < 0.0001, *** P < 0.001, * P < 0.05, and ns means not significant
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