Gel from Scientific Research

Ubiquitination assays showed that USP15 deubiquitinates GPX2 at K187(K, lysine) site. Cells were transfected with mutant GPX2 protein with Flag-vector or Flag-USP15. The myc-Ub conjugated protein was pulled down by anti-HA antibody. The anti-myc antibody was used to detect the polyubiquitination level of GPX2 (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001)

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