Healthy bladder without tumour and without nanobots (same sample as in Supplementary Fig. 4o-x ). Each tissue undergoes identical processing and visualization ( n = 1). Samples are imaged in autofluorescence and with V-LS (vertically-polarized light-sheet) and V-CAM (vertically-polarized detection) sLS. Left to right: single slice in mid-plane with autofluorescence (cyan) and processed mask (magenta) of internal tissue after removing internal lumen and digitally eroding an external layer of 500 m from the outer edge of the bladder; summed intensity of sLS signal inside the mask; single slice of sLS signal inside the mask; MIP of sLS signal inside the mask and two insets showing the same sLS signal with different look-up tables and intensity scales ( j ). Rows represent different animals and conditions. In g , the cavity inside the bladder was too thin and collapsed to be segmented, hence the mask includes the full volume (including the lumen), although residual cavity background does not contribute to the high intensities. Look-up table scales (‘Red Hot’ and ‘Inverted Red Hot’) and intensity limits shown in i , j apply to all panels. Scale bars, a , c , e , g , i , 1 mm; b , d , f , h , j insets, 200 m.
