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Overview of experimental design. EBs were generated in three replicates of six to nine human and chimpanzee iPSC lines. After two days in micropatterned plates, EBs were grown in low-adherence plates for three weeks before dissociating to single cells and sequencing with 10x scRNA-seq.
#Workflow#Illustration#Table Image#Experimental Design#Embryoid Bodies#iPSC Lines#Single-cell RNA sequencing
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