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Immunohistochemical analyses showing the distribution of H3R2me2s + cells of cortical organoids at day 50. The average expression of H4R3me2s of each cell was quantified in each vertical position bin (lower left). The normalized abundance of H3R2me2s + cells in each vertical position bin was calculated as in ( d ). CTRL, n = 49; OXR1 ΔEx18 , n = 53 from two independent batches. Values represent mean ± SEM, * p < 0.05, ** p < 0.01, ***p < 0.001, Student’s t test. In all confocal images, the scale bar is 60 μm and the nuclei were visualized using DAPI (blue)
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