Scatter Plot from Scientific Research

t-SNE and trajectory information of CD4 + T cell subtypes found by Slingshot analysis on reconstructed-hq data. While uncorrected data shows no clear cell type clustering (see B ), reconstructed data shows a clear grouping of cell types. Trajectories were calculated using CD4_naive as starting point and TH2, TH17, TH1, Active_TREG, CD4_CM as endpoints. Lineage1 indicates TH1, Lineage2 TH17, Lineage3 Active_TREG, Lineage4 TH2, and Lineage5 Effector cell differentiation.

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