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The pH profile of DR11-4T12. Cleavage reactions were carried out at 23 C in 35 % DMSO, 300 mM Na+, and 30 mM Mg2+at specific pH made with a relevant buffering agent at 50 mM (see Figure S9 for dPAGE analysis of the representative reactions). Error bars represent mean+/-SD for at least two independent experiments.
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