(Top left) Schematic of wound healing experiment, where epidermis and upper HF are denuded, leaving HFSC bulges to both re-epithelialize and regenerate hair. HFSCs were lineage-marked in telogen prior to feeding control or -ser/gly chow and wounding. (Top right) Re-epithelialization time course analyzed by IMF. Mean ± SEM are shown withn= 6–20 mice per group per time point (n= 11–15 mice for 1 dpw,n= 3 mice for 2 dpw, 17–20 mice for 3 dpw,n= 6 mice for 5 dpw, andn= 6–8 mice for 7 dpw).pvalue reflects a sigmoidal model with an extra sum of squares F-test. (Bottom left) Representative IMF of re-epithelialization across 1–7 dpw. Green denotes YFP-lineage-traced cells from SOX9+HFSCs. Scale bar, 50 μm. (Bottom right) Quantifications of HF downgrowth at 7 dpw. Each data point denotes one mouse. Skin epithelium is demarcated by white dotted lines. The scab-epidermal interface is denoted by yellow dashed lines. SeeFigure S1for additional experiments. ****denotes apvalue of < 0.0001,***denotes apvalue of < 0.001, and*denotes apvalue of < 0.05. Error bars are mean ± SEM.
