UCSC browser view of total reads from CTCF, H3K4me3, H3K27ac, H3K36me3, and H3K27me3 CUT&RUN in WT and Del-Firre Patski cells. Note that allelic analysis was not possible due to limited read coverage and short reads (36nt). Considering that expression of Car5b on the Xi but not on the Xa is significantly increased in Del-Firre cells as shown by allelic RNA-seq in B , we infer that increases in CTCF (black arrows) and in active histone marks at the promoter (H3K4me3; red arrow), distal enhancer (H3K4me3 and H3K27ac; orange arrow), and 3’ region (H3K36me3 for elongation; dotted box) of Car5b are probably from the Xi allele. The decrease in H3K27me3 enrichment (dotted box) is also probably from the Xi allele, as suggested by loss of Xi-specific H3K27me3 upon Firre depletion [ 43 ]
