Foxp3Thy1.1mice at 16 weeks of age were injected with intravenous anti-CD45 antibody label prior to fluorescence-activated cell sorting (FACS) of Treg cells from blood, kidney, liver, pancreas, and LPL (n= 4) for analysis by single-cell TCR sequencing. Cells stained as positive for intravenous anti-CD45 antibody were excluded during sorting. Chord diagrams illustrate, for each tissue origin, the Treg cell TCR found to be shared between different tissues. For each tissue (main sector) of each mouse (sub-sector), the frequency-ranked TCR repertoire is shown as a bar plot. Lines joining 2 repertoires indicate a clonotype sharing between 2 tissues of the same mouse (green), the same tissue type between different mice (pink), or different tissue types in different mice (black).
