Individual results from T cells derived from each of the FlowCode retrogenic TCRs, across the assessed tissues. Top, absolute cell count of detected CD4+T cells for each clone (size) together with the frequency of Treg cells within the population (color; percentage listed on each sample). Frequencies are calculated from the aggregate of lymphoid (spleen, lymph node [LN]), non-lymphoid (kidney, lung, liver, pancreas), and gut (LPL) samples. Middle, for each retrogenic TCR clone, a pie chart visualizing the distribution of cells within the lymphoid, non-lymphoid, and gut compartments. Bottom, for non-lymphoid tissue Treg cells from each retrogenic TCR clone, a pie chart visualizing the distribution of cells within the non-lymphoid tissues. See alsoFigures S5S7andData S2.