The TPR and TNR of FlowSig output for a – c, respectively. We considered the effect of additional perturbation data and the effect of applying our biological flow model to constrain edges. In d – f, plots were generated over 500 simulations. Light blue boxes indicate the cases when total receptor expression (free plus bound receptor) was used as the inflow variable, while dark blue boxes indicate the cases when bound receptor expression was used as the inflow variable. Box plot whisker bounds are: d , minimum (TPR: control only 0.5, 0.5; control + perturbation: 0.5, 0.5; TNR: control only 0.56, 0.64; control + perturbation: 0.62, 0.72), maximum (TPR: control only 0.5, 0.5; control + perturbation: 0.5, 1.0; TNR: control only 0.79, 0.85; control + perturbation: 0.79, 0.85). Horizontal lines are defined by Q1 (TPR: control only 0.5, 0.5; control + perturbation: 0.5, 0.5; TNR: control only 0.56, 0.72; control + perturbation: 0.69, 0.77), median (TPR: control only 0.5, 0.5; control + perturbation: 0.5, 0.5; TNR: control only 0.69, 0.77; control + perturbation: 0.72, 0.77) and Q3 (TPR: control only 0.5, 0.5; control + perturbation: 0.5, 0.5; TNR: control only 0.77, 0.79; control + perturbation: 0.77, 0.79).
