p97 and aCt conjugates were fluorescently labelled to favour identification of the species by AGE (lanes 2 and 5). Individual compartments, each loaded with its corresponding protein (A(p97 FAM ), lane 3 and B(aCt Cy5 ), lane 6), were purified and combined into A(p97 FAM )/B(aCt Cy5 ) (yields in Supplementary Table 2 ). Successful formation of the target construct was verified by the co-localization of the fluorescence signals associated to the proteins and their co-migration with the AB origami structure (lane 7). TEM imaging of the protein-loaded structures (right) showed correct location of p97 (aCt was not visible due to the limited resolution of the microscope). Class averages are shown in the insets ( n =354 and n =680, for AB and A(p97)/B(aCt), respectively).
