Protein partitioning parameter, , calculated from confocal micrographs in b as the ratio between the fluorescence signal of the target proteins recorded within and outside the MLOs for individual synthetic cells (Supplementary Methods 3 ). Data are shown for the three systems in a and b , with and without protein-binding moieties. In the box plots, the central line marks the median, the box marks the Q1 (first quartile) Q3 (third quartile) interquartile range (IQR) and the whiskers enclose data points within Q1 1.5 IQR and Q3 + 1.5 IQR. Data points are relative to individual synthetic cells from a single field of view of one imaged sample ((i), (ii) and (iii), left) or two fields of view from two technical replicates ((iii), right). All data points are shown except for four outliers with > 5 in (i) and (iii), omitted for ease of visualization.
