Immunohistochemistry analysis of Reelin, NeuN and amyloid- ( h ) or phosphorylated tau ( i ) in 12-month-old App-KI mice ( h ) or 9-month-old Tau(P301S) transgenic mice ( i ), showing depletion of Reelin-positive neurons in the ECs of the KI and transgenic mice compared with those of the wild-type controls. Representative images (left) show Hoechst (blue); amyloid- ( h ; D54D2) or phosphorylated-tau ( i ) (green); NeuN (yellow); and Reelin (red). Scale bars, 100 m ( h and i ). Quantification (right) was performed using unpaired two-tailed Student’s t -tests; P = 0.0181 ( App -KI, h ; n = 7 ( App -KI) and n = 6 (wild type) mice) and P = 0.0005 (Tau(P301S), i ; n = 6 mice (Tau(P301S)) and n = 5 (wild type) mice). Data are mean s.e.m. ParaS, parasubiculum; PrS, presubiculum.
